Planar lipid bilayers in recombinant ion channel research
نویسندگان
چکیده
منابع مشابه
Single ion-channel current measurements from rat brain synaptosomes in planar lipid bilayers.
Because of their small size and inaccessibility, direct electrical measurements of ionic conductances from mammalian brain presynaptic nerve terminals have not been possible. Measurements using voltage-sensitive fluorescent dyes, however, indicate that the membrane potential of a preparation enriched in pinched-off and resealed presynaptic nerve terminals (synaptosomes) depends principally on K...
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Single channel currents of sodium channels purified from rat brain and reconstituted into planar lipid bilayers were recorded . The kinetics of channel gating were investigated in the presence of batrachotoxin to eliminate inactivation and an analysis was conducted on membranes with a single active channel at any given time . Channel opening is favored by depolarization and is strongly voltage ...
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The properties of the channel of the purified acetylcholine receptor (AChR) were investigated after reconstitution in planar lipid bilayers. The time course of the agonist-induced conductance exhibits a transient peak that relaxes to a steady state value. The macroscopic steady state membrane conductance increases with agonist concentration, reaching saturation at 10(-5) M for carbamylcholine (...
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To understand how plasma membranes may limit water flux, we have modeled the apical membrane of MDCK type 1 cells. Previous experiments demonstrated that liposomes designed to mimic the inner and outer leaflet of this membrane exhibited 18-fold lower water permeation for outer leaflet lipids than inner leaflet lipids (Hill, W.G., and M.L. Zeidel. 2000. J. Biol. Chem. 275:30176-30185), confirmin...
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Strong external electric field can destabilize membranes and induce formation of pores thus increasing membrane permeability. The phenomenon is known as membrane electroporation, sometimes referred to also as dielectric breakdown or electropermeabilization. The structural changes involving rearrangement of the phospholipid bilayer presumably lead to the formation of aqueous pores, which increas...
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ژورنال
عنوان ژورنال: Methods
سال: 2018
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2018.03.003